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Non-separation biochemical methods, mainly using spectrophotometric or spectrofluorimetric detection, are widely employed for their sensitivity, but usually lack specificity due to interference of other thiols (e.g., Cys, homocysteine, gGC, CG, protein thiols) [35,36,37]

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Whilst the resulting fragment hits would be expected to bind with a weaker affinity than HTS hits due to their smaller size, they can show a comparatively better ligand efficiency 25

Nature 368 , 502 (1994)

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